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Outbred nude mice are immunodeficient tumor transplant hosts, and the standard in vivo model for drug efficacy testing in oncology. Nude mice are athymic and hairless as a result of the recessive nu mutation. T cell precursors exist but development is blocked in the absence of a thymus, resulting in an immunodeficiency that permits transplantation of tumor cell xenografts.

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The two main defects of mice homozygous for the nude spontaneous mutation Foxn1 nuformerly Hfh11 nu are abnormal hair growth and defective development of the thymic epithelium. Nude mice are also athymic; homozygous nude mice lack T cells and suffer from a lack of cell-mediated immunity. Homozygous nude mice show partial defect in B cell development.

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More severely immunodeficient mice with loss of NK activity are needed to overcome this limitation. The K cell line was implanted subcutaneously to compare tumorigenicity between Nude-RJ mice and Nude mice. The cholangiocarcinoma mCherry expressing cell line KKU-M was implanted subcutaneously, and fluorescence intensity and tumor weight were measured. Subcutaneously-transplanted mCherry-transduced KKU-M cells were successfully detected with a fluorescence imager.

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Envigo uses well-established techniques to successfully produce time mated rats, mice, hamsters, and rabbits. We use an Impedence Meter for determining the stage of estrus in rats prior to breeding. Time mated rats and mice are determined by observation of a vaginal plug.

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A nude mouse is a laboratory mouse from a strain with a genetic mutation that causes a deteriorated or absent thymusresulting in an inhibited immune system due to a greatly reduced number of T cells. The phenotype main outward appearance of the mouse is a lack of body hair, which gives it the "nude" nickname. The nude mouse is valuable to research because it can receive many different types of tissue and tumor grafts, as it mounts no rejection response.

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Pups may be delivered in transit. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH 31M rodent diet and sterilized water ad lib, sterile contact bedding paper chip and a light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens.

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Maintained at industry-leading health standards and competitively priced, JAX nude mice are the best route to getting your experimental data faster. Mice homozygous for the Foxn1 nu spontaneous mutation lack a transcriptional factor necessary for development of thymic epithelia and normal hair development. Mutants are athymic, preventing normal T cell development. Lack of functional CD8 T cells leads to a loss of T cell-mediated cytotoxic activity.

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Megan M. If you are reading this you already know that the use of sentinel animals in research and production colonies is critical to facilitate health monitoring and maintain animal quality. A sentinel animal must have a strong but not excessive immune response to infectious agents, with the ability to seroconvert to viral agents.

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These mice possess a vestigial thymus which is incapable of producing mature T-cells as shown by a decreased lymphocyte population carrying the theta antigen and depleted 'thymus-dependent' areas in their peripheral lymphoid organs. However, they have T-cell precursors in their bone marrow. Nude mice lack 'thymosin', a thymic factor secreted by the epithelial cells of the thymus.

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