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The role of this subset in HIV-1 pathogenesis remains unclear. The lack of NK cell lineage-specific markers has complicated the study of minor NK cell subpopulations. A diverse array of activating and inhibitory receptors controls their function.

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These in vitro data suggest that ivacaftor has a similar effect on all CFTR forms with gating defects and support investigation of the potential clinical benefit of ivacaftor in CF patients who have CFTR gating mutations beyond GD. CFTR is an epithelial chloride channel that is composed of two membrane spanning domains that form the chloride channel pore, two nucleotide-binding domains NBD that bind and hydrolyze ATP, and a regulatory domain with several protein kinase A PKA phosphorylation sites [1]. Normally, CFTR is present at the epithelial cell surface where it allows chloride transport across the epithelial cell membrane to maintain salt, fluid, and pH balance in multiple organs [3].

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Gene gating is a phenomenon by which transcriptionally active genes are brought next to nuclear pore complexes NPCs so that nascent transcripts can quickly form mature mRNA associated with export factors. SAGA is a chromatin remodeling complex responsible for activating the transcription of certain inducible genes. Nucleoporins Nups are the main constituent proteins of NPCs and have been shown to play multiple roles in mediating several processes involved in gene gating.

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Human primary monocytes are heterogeneous in terms of phenotype and function, but are sub-divided only based on CD16 and CD14 expression. This long-standing CD16—CD14 classification system, however, has limitations as CD14 is expressed in a continuum, leading to subjectivity in delineating the non-classical and intermediate subsets; in addition, CD16 expression is unstable, making identification of the subsets impossible after in vitro culture or during inflammatory conditions in vivo. Hence, we aimed to identify the three monocyte subsets using an alternative combination of markers.

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Department of Developmental Neurobiology, St. Author contributions: J. Thalamocortical TC projections provide the major pathway for ascending sensory information to the mammalian neocortex.

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Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Clock gating CG is a widely used design method for reducing the dynamic power consumption in digital circuits.

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A model-based gating strategy is developed for sorting cells and analyzing populations of single cells. The strategy, named CCAST, for Clustering, Classification and Sorting Tree, identifies a gating strategy for isolating homogeneous subpopulations from a heterogeneous population of single cells using a data-derived decision tree representation that can be applied to cell sorting. Because CCAST does not rely on expert knowledge, it removes human bias and variability when determining the gating strategy.

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Thalamocortical TC projections provide the major pathway for ascending sensory information to the mammalian neocortex. Arrays of these projections form synaptic inputs on thalamorecipient neurons, thus contributing to the formation of receptive fields RFs in sensory cortices. Experience-dependent plasticity of RFs persists throughout an organism's life span but in adults requires activation of cholinergic inputs to the cortex.


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